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Fig. 6 | BMC Cancer

Fig. 6

From: New anti-cancer chemicals Ertredin and its derivatives, regulate oxidative phosphorylation and glycolysis and suppress sphere formation in vitro and tumor growth in EGFRvIII-transformed cells

Fig. 6

Ertredin did not directly inhibit EGFR kinase but stimulated ubiquitination of EGFR in NIH3T3/EGFRvIII cells. a Direct effect of Ertredin (red line) or AG1478 (blue line) on the tyrosine kinase of human EGFR. b Effect of Ertredin on autophosphorylation of EGFRvIII in NIH3T3/EGFRvIII cells. Cells (3 × 105 /mL, 5 mL) were seeded on ULAS 6-well plates and cultured for 22 h. Ertredin or AG1478 was then added to a final concentration of 1 μM. Cells were harvested at the indicated time after the addition of chemicals. Each cell lysate (16 μg of protein) was applied on 10 % SDS-PAGE followed by immunoblot analysis. c NIH3T3/EGFRvIII spheres in ULAS dishes were treated with Ertredin (final concentration, 2 μM), AG1478 (final concentration, 2 μM), or vehicle for 17 h in the presence of MG132 (50 μM). Lysates were prepared from the cells and subjected to immunoprecipitation with anti-EGFR antibody followed by western blot with respective antibodies as shown (lane 1–3). Input lanes indicate 3.7 % of lysate used in immunoprecipitation (lane 4–6). The triangles indicate the estimated position of EGFRvIII

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