Fig. 5

Ertredin induced apoptosis in vitro 3D-sphere and in vivo tumors of NIH3T3/EGFRvIII cells. a Cells with the addition of Ertredin (red line) or AG1478 (blue line) were seeded on ULAS plates (left) or tissue culture treated plates (right), followed by cultivation for 72 h. Caspase 3/7 activities were measured as described in Materials and Methods. b (Upper) Spheres were observed by phase contrast microscopy 72 h after seeding of cells on ULAS plates. (Lower) Apoptotic cells (brown) in paraffin sections were determined by TUNEL assay. Cells were cultured in medium with Ertredin (2 μM) (right) or vehicle (left). Apoptotic cells were stained brown. DNA counterstained with Methyl Green. c TUNEL staining for the detection of apoptotic cells in tumors isolated from mice treated with Ertredin (right) or control mice without Ertredin (left)