Fig. 3
Silibinin induced apoptosis through mitochondrial ROS in PC-3 cells. a PC-3 cells were treated with 150 μM silibinin for indicated times. MMP was determined using fluorescence dye DiOC6 by flow cytometry. b Protein expression was analyzed by western blotting with antibodies for pro-caspase-3 and PARP. β-Actin was used as a loading control. c Apoptosis was analyzed after treatment with 150 μM silibinin for 48 h with the presence or absence of 0.5 μM DPI by flow cytometry. d Protein expression was analyzed by western blotting. β-Actin was used as a loading control. Data are presented as mean ± SD (n = 3 in each group). * p <0.001 vs. the control group