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Fig. 8 | BMC Cancer

Fig. 8

From: 5-lipoxygenase mediates docosahexaenoyl ethanolamide and N-arachidonoyl-L-alanine-induced reactive oxygen species production and inhibition of proliferation of head and neck squamous cell carcinoma cells

Fig. 8

Exogenous transfection of plasmids expressing 5-LO promotes the anti-cancer action of DHEA and NALA in HNSCC cells. a Plasmids (0.5-1 μg) expressing 5-LO were transfected into SNU-1041 (LacZ expressing plasmid was used for controls). Thirty-six hours later cells were treated with DHEA and NALA (10 μM). At additional 48 h, cells were subjected to cell proliferation assay. Results are expressed as a percentage relative to control (% of control). P values were based on comparison with DHEA-treated group and NALA-treated group in LacZ (*P < 0.01, #P < 0.05). b Plasmids (0.5-1 μg) expressing LacZ and 5-LO were transfected into SNU-1041 cells. At 36 h, total cell lysates were prepared, and the expression of 5-LO was determined by western blotting. Data are presented as a representative of 3 separate experiments. c Plasmids (1 μg) expressing 5-LO were transfected into SNU-1041. Thirty-six hours later cells were treated with DHEA, NALA and AA (10 μM). At additional 18 h, cells were subjected to the DCFH2-DA assay to measure the change of ROS level. Results are expressed as a percentage relative to control (% of control). P values were based on comparison with DHEA-treated group and NALA-treated group in LacZ (*P < 0.005, #P < 0.01)

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