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Fig. 6 | BMC Cancer

Fig. 6

From: 5-lipoxygenase mediates docosahexaenoyl ethanolamide and N-arachidonoyl-L-alanine-induced reactive oxygen species production and inhibition of proliferation of head and neck squamous cell carcinoma cells

Fig. 6

5-LO inhibition as well as antioxidant treatment partially reversed DHEA- and NALA-inhibited cell proliferation. a Cells were treated with 20 μM of DHEA and NALA plus NAC (1 mM) or GSH (2 mM). At 72 h, cells were subjected to cell proliferation assay. Results are expressed as a percentage relative to control (% of control). P values were based on comparison with DHEA-treated group and NALA-treated group (*P < 0.001, #P < 0.01). b Cells were transfected at 200 nM doses of 5-LO siRNA (the siNC was used for negative control of siRNA). Forty-eight hours later cells were treated with DHEA or NALA (20 μM). At additional 18 h, cells were subjected to the DCFH2-DA assay to measure the change of ROS level. Results are expressed as a percentage relative to control (% of control). P values were based on comparison with DHEA-treated group and NALA-treated group in siNC (*P < 0.01, #P < 0.05)

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