Fig. 4
The anti-cancer effects of DHEA and NALA are not mediated by any products generated by the 5-LO pathway. a SNU-1041 and SNU-1076 were treated with AA, AEA, DHA, DHEA and NALA (20 μM). At 4 h, cells were subjected to the LTB4 EIA. Results are expressed as a percentage relative to control (% of control). b SNU-1041 cells were transfected with 200 nM of siRNA against 5-LO or si-NC and placed at once in the lower side of a transwell chamber. Then SNU-1041 cells (with no transfection) were seeded in the upper part and co-cultured for 48 h. Subsequently, cells were treated with 30 μM of DHEA or NALA for additional 48 h. Both cells (in upper and lower side) were separately applied to the cell proliferation assay. Results are expressed as a percentage relative to control (% of control). P values were based on comparison with control (*P < 0.01, **P < 0.05) or DHEA/NALA-treated group (#P < 0.005, ##P < 0.05)