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Fig. 3 | BMC Cancer

Fig. 3

From: Highly sensitive detection of the PIK3CAH1047R mutation in colorectal cancer using a novel PCR-RFLP method

Fig. 3

a: Detection specificity of PCR-RFLP. Synthetic oligonucleotide sequences containing wild-type PIK3CA (CAT) and mutant-type PIK3CA (CGT or CTT) were subjected to PCR-RFLP. b: Detection sensitivity of PCR-RFLP. The PIK3CAH1047R mutant plasmid was spiked into wild-type plasmid at different ratios, and analyzed by PCR-RFLP method (top) and direct sequencing (bottom). M: DL500 DNA marker

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