Fig. 2
Detection of the PIK3CAH1047R mutation in CRC cell lines using PCR-RFLP. a: Detection of four CRC cell lines with known PIK3CA gene status using PCR-RFLP. The fragment of 126-bp was amplified from the cells’ gDNA, digested with FspI, and then electrophoresed in a 3 % agarose gel. The FspI digestion of wild-type PIK3CA yields two bands of 96- and 30-bp, while the mutant-type remains intact (126 bp). b: The detection of six CRC cell lines with unknown PIK3CA gene status by PCR-RFLP (top) and direct sequencing (bottom). M: DL500 DNA marker