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Fig. 5 | BMC Cancer

Fig. 5

From: Everolimus downregulates estrogen receptor and induces autophagy in aromatase inhibitor-resistant breast cancer cells

Fig. 5

Everolimus treatment inhibits estrogen receptor α expression and activity. a ER transcriptional activity after 24 h treatment of MCF-7, MCF-7:5C and MCF-7:2A cells with 20 nM everolimus (EVE) and 1 μM fulvestrant (FUL) is demonstrated using an ERE luciferase reporter. b Corresponding expression of estrogen receptor α (ERα) after 48 h of treatment is shown by western blot. c RT-PCR demonstrates the impact of 20 nM everolimus on ERα mRNA production in all three cell lines after 24 h. d The effect of everolimus treatment on mTOR phosphorylation as well as HSP90 and ERα expression is shown. e Immunofluorescent confocal microscopy illustrates the impact of 20 nM everolimus on ERα expression and localization (green). Cell nuclei are stained with DAPI (blue). Bar graphs represent means from two independent experiments analyzed in quadruplet and are displayed as mean ± SD. ** p < 0.01

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