Fig. 4

Everolimus targets the mTOR/Akt pathway. AI-sensitive MCF-7 and AI-resistant MCF-7:5C and MCF-7:2A cells were treated with 25, 50 and 100nM everolimus. Phosphorylation of mTOR, AKT, p70s6K, 4EBP1 and total protein levels are shown at 30mins, 60mins, 12 and 24 h. Images are representative of three independent experiments