Fig. 2

Everolimus induces G1 arrest. a MCF-7, MCF-7:5C and MCF-7:2A cells were treated for 24 h with 20 nM everolimus or vehicle and then harvested by trypsinization. Samples were fixed with methanol, stained with propidium iodide and analyzed by flow cytometry. The percent of cells in each phase of the cell cycle from a representative experiment are indicated in pie charts. b Samples from cells treated for 24, 48 and 72 h are summarized in the table. Values are means from three independent experiments analyzed in duplicate and are displayed as mean ± SD. ** p < 0.01. c Cyclin D1 and p21 expression in MCF-7, MCF-7:5C and MCF-7:2A cells following treatment with everolimus (20 nM) for 48 and 72 h