Fig. 1
Everolimus inhibits the proliferation of AI-sensitive and AI-resistant breast cancer cells. a MCF-7, MCF-7:5C and MCF-7:2A cells were treated with 20 nM everolimus or vehicle (control) for 72 h. The percent of viable cells after everolimus treatment was determined by cell viability assay and compared to control. IC50 values for each of the cell lines were determined by nonlinear regression on normalized values. Values represent means of three experiments conducted in quadruplet. b MCF-7, MCF-7:5C and MCF-7:2A cells were seeded in 24-well plates and treated with 20 nM everolimus or vehicle after overnight acclimatization (Day 0). The percentage of viable cells was determined at 24, 48, 72 and 96 h post treatment by comparison to vehicle treated cells. c Cells were seeded in single cell suspension and allowed to proliferate for 9 days in the presence of 20nM everolimus or vehicle (control). The plates were photographed at 1X magnification (left panel) and the number of colonies and colony size were quantified using Image J (right panel). Bar graphs represent the data from three independent experiments in triplicate and values are mean ± SD. ** p < 0.01