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Table 3 Expression of Ad receptor molecules after ZOL treatments

From: Combination of a third generation bisphosphonate and replication-competent adenoviruses augments the cytotoxicity on mesothelioma

Cells Ab ZOL treatment Mean fluorescent intensity (average ± SE) % radio (average ± SE)
MSTO-211H 2nd Ab alone (−) 12.42 ± 0.07  
(+) 13.44 ± 0.07 108.19 ± 0.51
CAR (−) 95.21 ± 2.04  
(+) 95.86 ± 0.09 100.69 ± 0.09
Integrin αvβ3 (−) 13.59 ± 0.02  
(+) 14.67 ± 0.04 107.92 ± 0.04
Integrin αvβ5 (−) 15.64 ± 0.10  
(+) 18.12 ± 0.03 115.88 ± 0.64
NCI-H28 2nd Ab alone (−) 11.39 ± 0.03  
(+) 11.55 ± 0.86 101.38 ± 0.79
CAR (−) 127.26 ± 0.31  
(+) 119.29 ± 0.69 93.73 ± 0.03
Integrin αvβ3 (−) 1006.61 ± 1.24  
(+) 718.29 ± 2.33 71.36 ± 0.12
Integrin αvβ5 (−) 44.01 ± 0.09  
(+) 22.01 ± 0.03 50.01 ± 0.13
  1. Cells untreated or treated with ZOL (MSTO-211H: 10 μM, NCI-H28: 80 μM) for 48 h were subjected to flow cytometry to detect expression of the cellular receptors of type 5 Ad. Means fluorescence intensity was expressed as an arbitrary unit and influence of ZOL on the expression levels was expressed as a percent ratio based on respective ZOL-untreated cases as a control (n = 3)