Fig. 2
Tasquinimod reduces the recruitment of Ly6Chi cells to 4 T1 tumors. BALB/c mice were inoculated with 105 4 T1 cells s.c. Tasquinimod (25 mg/kg/day) was given in the drinking water starting on day 4 post-inoculation and BrdU (2 mg) was injected i.p. starting at day 5 post-inoculation. Three injections of BrdU were given with regular intervals and the last injection 14 h before sacrifice. Spleens and tumors were collected 7 days post-inoculation and analyzed by flow cytometry. a, Representative FACS plots showing the efficiency of BrdU labeling of Ly6Chi and Ly6Ghi cells in a 4 T1 tumor. b, Number of tumor BrdU+ Ly6Chi and BrdU+ Ly6Ghi cells gated from CD11b+ CD45.2+ cells as in Fig. 1c. c, Number of total tumor Ly6Chi, Ly6Ghi and SiglecF+ cells. d, Number of splenic BrdU+ Ly6Chi, BrdU+ Ly6Ghi and BrdU+ SiglecF+ cells in 4 T1 tumor-bearing mice. Pooled data (indicated by open and filled symbols, respectively) from two independent experiments are shown (ctrl, n = 14; tasq, n = 14). n.s. = not significant, ***P < 0.001, Mann–Whitney U-test