Skip to main content

Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Table 4 Analytical variation presented as standard deviations of the 40-ΔΔCq values (SD) with 95 % confidence intervals (CI) for LightCycler 480 instrument II, Roche

From: Technical validation of an RT-qPCR in vitro diagnostic test system for the determination of breast cancer molecular subtypes by quantification of ERBB2, ESR1, PGR and MKI67 mRNA levels from formalin-fixed paraffin-embedded breast tumor specimens

LightCycler 480 II   Study 1 Study 2
   Inter-Site Inter-Run Inter-run Intra-Run Inter-Extraction Intra-Extraction
     Device 1 (site 1) Device 2 (site 2) Device 3 (site 2) Device 4 (site 3)
ERBB2 SD 0.20 0.21 0.21 0.18 0.22 0.21 0.20 0.08 0.16
95 % CI 0.15–0.30 0.19–0.23 n.a. n.a. n.a. n.a. n.a. 0.05–0.19 0.14–0.19
ESR1 SD 0.18 0.27 0.26 0.27 0.21 0.31 0.30 0.52 0.55
95 % CI 0.13–0.29 0.24–0.30 n.a. n.a. n.a. n.a. n.a. 0.39–0.79 0.48–0.64
PGR SD 0.14 0.15 0.14 0.12 0.19 0.16 0.22 0.07 0.17
95 % CI 0.11–0.22 0.14–0.17 n.a. n.a. n.a. n.a. n.a. 0.04–0.26 0.15–0.20
MKI67 SD 0.20 0.18 0.18 0.14 0.15 0.23 0.20 0.05 0.17
95 % CI 0.16–0.30 0.16–0.20 n.a. n.a. n.a. n.a. n.a. 0.03–0.68 0.15–0.20
  1. All data using models without day (inter-day variance is completely explained (covered) by inter-run variance). The calculations of inter- and within-section SDs are derived from a single instrument (study 2), due to the use of pooled samples in study 1