Fig. 3

MiR-944 alters cytoskeleton and focal adhesions. MDA-MB-231 and MCF-7 cells were treated for indirect immunofluorescence with rhodamine phalloidin to visualize F-actin (red) or with α-actinin1 antibody labeled with FITC (green). Nuclei were counterstained with DAPI (blue). (a) Phase contrast and immunofluorescence images show actin organization in non-transfected (control) and miR-944 expressing MDA-MB-231 (top panels) and (c) MCF-7 cells (bottom panels). Arrowheads indicate representative actin-rich membrane ruffling (MR); asterisk indicates filopodia (f). (b) Representative x-z confocal images of α-actinin-1 (green) and F-actin (red) organization in MDA-MB-231 (top panels) and (d) MCF-7 cells (bottom panels) non-transfected (control) or transfected with miR-944 precursor