Fig. 6

Pathological findings of human muscle-derived gene expression at the injection site. Immunohistochemistry showing human muscle-derived gene expression (SIX1) in 3 normal subcutaneous regions (upper) and the scar-like, slightly protruding region (middle). The human muscular region in subcutaneous tissue (bottom left) and the murine subcutaneous region (bottom right), including muscle, were used as a positive control and a negative control, respectively. Scar-like formation (HE stain; middle left) induced by injection retained GFP expression (middle center) and weak human SIX1 expression in murine tissue (middle right). The representative region for immunostaining is denoted by a rectangle (top left), and arrows denote moderate human SIX1 expression. The arrow shows the murine muscle region without human SIX1 staining (bottom left)