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Fig. 3 | BMC Cancer

Fig. 3

From: Tumor-suppressive effects of atelocollagen-conjugated hsa-miR-520d-5p on un-differentiated cancer cells in a mouse xenograft model

Fig. 3

An in vivo study to assess the topical effects of 520d-5p-conjugated atelocollagen on cancer tissues. a, b After we generated GFP-expressing HLF (HLF/GFP) or HMV-I (HMV-I/GFP) cells using lentiviral constructs (top for each), the therapeutic effects of 520d/atelocollagen on the resulting GFP-expressing tumors in mice were examined. The average fluorescence intensity in tumors was measured, and representative data are presented through week 8 (bottom of each panel). GFP expression was maintained during the observation period in both studies. Dotted lines indicate tumor growth in controls. c Overviews of the in vivo study using 520d/atelocollagen in a mouse xenograft model (top, HLF; bottom, HMV-I). In the group that was administered the 520d/atelocollagen complex, tumor volume was significantly suppressed relative to the control group. *, P < 0.01 by the Mann-Whitney U test. The tumor volume of HLF cells from 11 to 15 weeks or of HMV-I cells from 9 to 12 weeks was analyzed and compared with control or scrambled data at 10 weeks or 8 weeks, respectively. The arrow indicates the timing of the doses that were administered to each group from 3 to 9 weeks (c) and from 3 to 7 weeks (d). : injection of complex (once a week); ▲: atelocollagen alone; : scramble/atelocollagen; ■: miR-520d-5p/atelocollagen (cases with suppressive growth); □: miR-520d-5p/atelocollagen (cases with tumor disappearance); ✝: mice were sacrificed; CI: confidence interval

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