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Table 3 FISH spots detected per CTC in a patient with an ERG-rearranged tumor depending on the number of z-stacks

From: Method for semi-automated microscopy of filtration-enriched circulating tumor cells

CTC ID Number of FA-FISH spots
  5 z-stacks 10 z-stacks 15 z-stacks 20 z-stacks 25 z-stacks 30 z-stacks
1 5 9 2 10 16 22
2 23 32 22 32 33 36
3 4 12 6 15 16 16
4 11 20 10 24 30 32
5 10 6 12 16 14 16
6 26 14 2 30 31 45
7 34 4 2 39 59 59
8 15 4 4 27 31 31
9 14 8 2 31 33 34
10 6 2 16 26 26 31
11 2 2 4 15 16 22
12 14 32 2 60 58 61
13 13 0 20 28 28 28
14 10 10 12 35 32 35
15 17 10 10 56 53 65
16 16 6 8 28 30 32
17 6 6 10 14 15 16
18 5 14 2 14 14 20
19 8 4 6 16 26 31
20 30 20 26 52 52 44
21 4 16 6 12 18 27
22 7 n.ia 6 30 29 30
%b 0 % 0 % 0 % 23 % 23 % 95 %
  1. Abbreviations: CTC circulating tumor cell; ERG v-ets avian erythroblastosis virus E26 oncogene homolog; FISH fluorescence in situ hybridization; n.i. non-interpretable
  2. aNumber of FISH spots is uncountable due to non-optimal focus in the DAPI channel
  3. bPercentage of cases where a higher number of FISH spots were observed
  4. The numbers in bold correspond to the highest number of spots for this CTC