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Table 3 FISH spots detected per CTC in a patient with an ERG-rearranged tumor depending on the number of z-stacks

From: Method for semi-automated microscopy of filtration-enriched circulating tumor cells

CTC ID

Number of FA-FISH spots

 

5 z-stacks

10 z-stacks

15 z-stacks

20 z-stacks

25 z-stacks

30 z-stacks

1

5

9

2

10

16

22

2

23

32

22

32

33

36

3

4

12

6

15

16

16

4

11

20

10

24

30

32

5

10

6

12

16

14

16

6

26

14

2

30

31

45

7

34

4

2

39

59

59

8

15

4

4

27

31

31

9

14

8

2

31

33

34

10

6

2

16

26

26

31

11

2

2

4

15

16

22

12

14

32

2

60

58

61

13

13

0

20

28

28

28

14

10

10

12

35

32

35

15

17

10

10

56

53

65

16

16

6

8

28

30

32

17

6

6

10

14

15

16

18

5

14

2

14

14

20

19

8

4

6

16

26

31

20

30

20

26

52

52

44

21

4

16

6

12

18

27

22

7

n.ia

6

30

29

30

%b

0 %

0 %

0 %

23 %

23 %

95 %

  1. Abbreviations: CTC circulating tumor cell; ERG v-ets avian erythroblastosis virus E26 oncogene homolog; FISH fluorescence in situ hybridization; n.i. non-interpretable
  2. aNumber of FISH spots is uncountable due to non-optimal focus in the DAPI channel
  3. bPercentage of cases where a higher number of FISH spots were observed
  4. The numbers in bold correspond to the highest number of spots for this CTC