Skip to main content

Table 2 FISH spots detected per CTC in a patient with a ROS1-rearranged tumor depending on the number of z-stacks

From: Method for semi-automated microscopy of filtration-enriched circulating tumor cells

CTC ID

Number of FA-FISH spots

 

5 stacks

10 stacks

15 stacks

20 stacks

25 stacks

30 stacks

1

9

n.ia

6

14

14

30

2

4

10

10

10

10

10

3

12

22

18

28

28

28

4

3

2

n.ia

13

14

14

5

4

4

4

4

4

4

6

26

28

26

34

34

34

7

6

15

11

20

28

28

8

36

42

48

52

62

62

9

10

14

18

20

30

30

10

13

20

22

26

30

30

11

2

12

12

13

14

14

12

6

6

8

10

14

14

13

4

6

6

8

14

14

14

8

13

20

32

32

32

15

10

14

14

16

16

16

16

10

12

13

16

16

16

17

2

6

4

15

16

16

18

4

11

16

25

25

25

19

20

33

26

46

55

64

20

12

14

12

14

15

14

21

11

n.ia

20

24

28

28

22

10

12

12

20

19

12

%b

4 %

9 %

9 %

41 %

86 %

91 %

  1. Abbreviations: CTC circulating tumor cell; FISH fluorescence in situ hybridization; n.i. non-interpretable; ROS1 c-ros oncogene 1
  2. aNumber of FISH spots is uncountable due to non-optimal focus in the DAPI channel
  3. bPercentage of cases where a higher number of FISH spots were observed
  4. The numbers in bold correspond to the highest number of spots for this CTC