Skip to main content

Advertisement

Table 2 FISH spots detected per CTC in a patient with a ROS1-rearranged tumor depending on the number of z-stacks

From: Method for semi-automated microscopy of filtration-enriched circulating tumor cells

CTC ID Number of FA-FISH spots
  5 stacks 10 stacks 15 stacks 20 stacks 25 stacks 30 stacks
1 9 n.ia 6 14 14 30
2 4 10 10 10 10 10
3 12 22 18 28 28 28
4 3 2 n.ia 13 14 14
5 4 4 4 4 4 4
6 26 28 26 34 34 34
7 6 15 11 20 28 28
8 36 42 48 52 62 62
9 10 14 18 20 30 30
10 13 20 22 26 30 30
11 2 12 12 13 14 14
12 6 6 8 10 14 14
13 4 6 6 8 14 14
14 8 13 20 32 32 32
15 10 14 14 16 16 16
16 10 12 13 16 16 16
17 2 6 4 15 16 16
18 4 11 16 25 25 25
19 20 33 26 46 55 64
20 12 14 12 14 15 14
21 11 n.ia 20 24 28 28
22 10 12 12 20 19 12
%b 4 % 9 % 9 % 41 % 86 % 91 %
  1. Abbreviations: CTC circulating tumor cell; FISH fluorescence in situ hybridization; n.i. non-interpretable; ROS1 c-ros oncogene 1
  2. aNumber of FISH spots is uncountable due to non-optimal focus in the DAPI channel
  3. bPercentage of cases where a higher number of FISH spots were observed
  4. The numbers in bold correspond to the highest number of spots for this CTC