Fig. 4
From: Method for semi-automated microscopy of filtration-enriched circulating tumor cells
Detection of ALK-rearrangement and gain of ALK-native copies in CTCs from a patient with an ALK-rearranged tumor using the multi-exposure protocol. ALK-gene status was tested in filtration enriched-CTCs by combined fluorescent staining and fluorescent in situ hybridization (FISH). FISH signals were captured with the multi-exposure protocol and 9 out of the 27 possibilities are shown. Images of best exposure level for red and green signals are framed in yellow. a Detection ALK-rearrangement. Green and red arrows indicate the break apart signal. b Detection of gain of ALK-native copies. Scale: white bars = 10 μm