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Fig. 2 | BMC Cancer

Fig. 2

From: Method for semi-automated microscopy of filtration-enriched circulating tumor cells

Fig. 2

Scanning and image gallery of A459 cells spiked in normal blood and filtered by ISET. a Images of the whole ISET filter (left panel). Grey windows and right panels show DAPI/CD45 APC and DAPI/CD45 APC/Epithelial markers AF488/Mesenchymal markers AF546 channels. b Zoom in images for DAPI/CD45 APC/Epithelial markers AF488/Mesenchymal markers AF546 channels. The image gallery of A549 cells and cell characteristics are shown. Cell categories after automated selection of CD45 cells and manual review of images are shown. The white arrow indicates an A549 cell, orange arrows indicate CD45+ cell into pores, the blue arrow a CD45+ cell outside a pore. Abbreviations: Avg_Int, DAPI average intensity; Avg_Int_Bg, background average intensity, Sec_Avg_Int, CD45 average intensity; Ter_Avg_Int, AF488 average intensity; Quat_Avg_Int, AF546 average intensity

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