Fig. 2
From: Method for semi-automated microscopy of filtration-enriched circulating tumor cells
Scanning and image gallery of A459 cells spiked in normal blood and filtered by ISET. a Images of the whole ISET filter (left panel). Grey windows and right panels show DAPI/CD45 APC and DAPI/CD45 APC/Epithelial markers AF488/Mesenchymal markers AF546 channels. b Zoom in images for DAPI/CD45 APC/Epithelial markers AF488/Mesenchymal markers AF546 channels. The image gallery of A549 cells and cell characteristics are shown. Cell categories after automated selection of CD45− cells and manual review of images are shown. The white arrow indicates an A549 cell, orange arrows indicate CD45+ cell into pores, the blue arrow a CD45+ cell outside a pore. Abbreviations: Avg_Int, DAPI average intensity; Avg_Int_Bg, background average intensity, Sec_Avg_Int, CD45 average intensity; Ter_Avg_Int, AF488 average intensity; Quat_Avg_Int, AF546 average intensity