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Fig. 8 | BMC Cancer

Fig. 8

From: Coalition of Oct4A and β1 integrins in facilitating metastasis in ovarian cancer

Fig. 8

Immunohistochemical analysis of Glut-1 and CK7 expression in Oct4A knockdown xenografts. a Representative immunohistochemistry staining images of debulked mouse xenografts for the expression of Glut-1 and CK7. Images are set at 200x magnification and scale bar represents 50 μM. b-c Quantification of Glut-1 and CK7 staining was determined by using Image J software recognizing DAB intensity. Variations in staining were determined by subtracting the negative control DAB reading from the DAB reading of the protein of interest for each xenograft. Data is presented as the mean ± SEM of staining intensity (n = 4/group). Significant variations between Oct4A KD groups and vector control was determined by Student’s t-test *P < 0.05

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