Fig. 6
From: Coalition of Oct4A and β1 integrins in facilitating metastasis in ovarian cancer
MMP9 and MMP2 secretion by HEY Oct4A KD monolayer cells. a Gelatin zymography analysis of MMP9 and MMP2 secretion by HEY Oct4A KD cells was performed on a 10 % polyacrlamide gel containing 1 % gelatine. Pro-MMP9 (~92 kDa) and Pro-MMP2 (~72 kDa) gelatinolytic activity is indicated by clear banding. b-c Densitometric analysis of gelatin zymography was performed to determine secreted Pro-MMP9 and Pro-MMP2 activity. Enzymatic activity is expressed as the intensity of the Pro-MMP9 and Pro-MMP2 bands of interest and presented as the mean ± SEM from three independent experiments. Significance is indicated by *P < 0.05 as determined by One-Way ANOVA using Dunnett’s Multiple Comparison post-test against HEY vector control cells