Fig. 5

Expression of integrin α2, α5 and β1 subunits in HEY Oct4A knockdown monolayer cells as determined by flow cytometry. a Flow cytometric analysis was used to examine integrin α2, α5 and β1 subunit expression in monolayer Oct4A KD cells. Cells were cultured, collected and incubated with respective primary antibodies or negative IgG control before being detected using a secondary goat anti-mouse IgG conjugated with phycoerythrin. Vector control cells are represented by solid black peaks and broken peaks are indicative of Oct4A KD cells as indicated. The filled histogram denotes the negative control IgG. b Semi-quantitative analysis of flow cytometry results. Results are expressed as the difference between the arbitrary fluorescence expression of the integrin of interest and the negative control IgG and presented as the mean ± SEM of four independent experiments (except integrin α5, n = 3). Significance is indicated by *P < 0.05, **P < 0.01 and ***P < 0.001 as determined by One-Way ANOVA with Dunnett’s Multiple Comparison post-test compared to vector control. c The adhesive ability of Oct4A KD cells on collagen and fibronectin was determined by 24 h adhesion assay. The number of adhered cells was estimated by measuring the optical absorbance at OD_550nm with the SpectraMax190 Absorbance Microplate Reader. Data is expressed at the mean ± SEM of three independent experiments performed in triplicate. Significance between Oct4A KD cells and vector control cells per ECM group is indicated by ***P < 0.001 as determined by Two-Way ANOVA and Bonferroni post-test