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Fig. 4 | BMC Cancer

Fig. 4

From: Coalition of Oct4A and β1 integrins in facilitating metastasis in ovarian cancer

Fig. 4

β1 and α5 integrin subunit expression and localisation in HEY Oct4A knockdown spheres. Expression and localisation of β1 and α5 integrin subunits in 18 day Oct4A KD spheres was evaluated using immunofluorescence staining. Adhered spheres were immunostained using mouse monoclonal antibodies for integrin a β1 or integrin d α5 and visualised using the secondary Alexa 488 fluorescent labelled antibody (green). Nuclear staining was visualised using DAPI staining (blue). Magnification is set at 100x for top and middle image panels and 200x for images in the bottom panel. Scale bar is set at 50 μM. Mean fluorescence intensity of b β1 and e α5 staining is presented as the mean ± SEM of respective integrin expression standardised to DAPI from 3 independent experiments. c Quantitative real-time PCR analysis was used to determine the expression of integrin β1 subunit in non-adherent 18 day Oct4A KD spheres. Relative quantification of mRNA expression was standardised to 18S housekeeping gene and normalised to the vector control. Data is expressed as the mean fold change ± SEM from three independent samples assessed in triplicate. Significance is indicated by *P < 0.05 and **P < 0.01 as determined by One-Way ANOVA using Dunnett’s Multiple Comparisons post test against the vector control

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