Skip to main content
Fig. 2 | BMC Cancer

Fig. 2

From: Coalition of Oct4A and β1 integrins in facilitating metastasis in ovarian cancer

Fig. 2

Sphere forming abilities and adhesion profiles of spheroids produced from epithelial ovarian cancer cell lines. a 1 x 105 OVCAR5, SKOV3, OVCA433 and HEY cells were plated on ultra-low attachment plates in the presence of complete growth medium and sphere formation was monitored over 18 days. Images are representative of 3 independent experiments and taken from a section of a 6 well plate well using an inverted phase contrast microscope at 100x magnification. Scale bar represents 100 μM. b 18 day spheroids derived from OVCAR5, SKOV3, OVCA433 and HEY cells were collected, plated and cultured on plastic for a further 24 h. Images of adhered spheres were taken using an inverted phase contrast microscope at 100x magnification. Scale bar represents 100 μM. Adhered spheroids were also assessed by crystal violet staining to determine colony formation. Images are that of an entire well of a 6 well plate and representative of 3 experiments prepared in triplicate

Back to article page