Fig. 5
From: Context dependent regulatory patterns of the androgen receptor and androgen receptor target genes
Forced Androgen Receptor expression induces target gene expression. a Phase-contrast of cells grown in Matrigel-overlay culture at day 12 with 10 nM testosterone. b-c Agilent microarray gene expression data were analyzed using SAM in the J-Express software to find fold change upregulation (positive numbers) or downregulation (negative numbers) of the shown genes in EP156T-AR cells stimulated by 10 nM Testosterone for 14 days compared to EP156T-AR cells grown in androgen free medium. d-g RT-qPCR of EP156T-AR cells in androgen free or medium with 10 nM testosterone in 2D or Matrigel-overlay culture. d  AR, e  KLK3/ACTB ratio, f  TP63 and g  TMPRSS2. Error bars show 95 % confidence intervals. RQ = relative quantity. Scale bars 200 μm. N.D. = not detected