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Fig. 4 | BMC Cancer

Fig. 4

From: Epigenetic silencing of serine protease HTRA1 drives polyploidy

Fig. 4

Increased proliferation after HTRA1 depletion. a HTRA1 expression measured by qRT-PCR (n = 2 independent experiments, each assayed in triplicate) and Western blots. Upper panel, Htra1 +/+ and Htra1 −/− MEFs (two-tailed Mann–Whitney U test, p-value <0.0001). Lower panel, supernatants of parental or stably transfected SW480 cells (Par, untransfected SW480; overprod., SW480 stably transfected with plasmid p50HTRA1; EV ctrl., corresponding empty vector control; shD3, downregulated via shHTRA1 D3; shS8, downregulated via shHTRA1 S8; two-tailed Mann–Whitney U test, p-values: EV ctrl. = 0.3776, shD3 = 0.005, shS8 = 0.005). Actin was used as loading control for cell lysates (right). b Growth curves. Left panel, MEFs. 3x105 cells were placed in 6 cm dishes and numbers of cells were counted at the time points indicated. Right panel, SW480 derivatives. 2.5x104 cells were placed into 6-wells and numbers of cells were counted at time points indicated. (n = 2 independent experiments, standard deviations are indicated). c Mean diameter and volume of Htra1 +/+ and Htra1 −/− MEFs (unpaired two-tailed t-test, p-value <0.0001)

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