Fig. 2From: Epigenetic silencing of serine protease HTRA1 drives polyploidy Htra1 expression and methylation of the Htra1 promoter in polyps. Mean normalized expression (MNE) of Htra1 in polyps isolated from Apc Min+ mice was determined by qRT-PCR. Methylation of the murine Htra1 promoter (−252 bp to −12 bp) was determined by sequencing 3 independent clones of bisulfite treated DNA isolated from the same polyps that were used for measuring mRNA levels by qRT-PCR. Controls are four independent samples taken from large intestineBack to article page