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Fig. 4 | BMC Cancer

Fig. 4

From: Metformin treatment modulates the tumour-induced wasting effects in muscle protein metabolism minimising the cachexia in tumour-bearing rats

Fig. 4

Expression of proteins of ubiquitin-proteasome pathway are increased in Walker 256 tumour-bearing rats and metformin is able to restore similar levels as C group. a Representative image from at least four independent experiments. Expression of proteasome 20S subunit, the 32 KDa subunit (b) and the 28 KDa subunit (c) were analyzed by immunoblotting. Regulatory proteasome subunits 19S (d) and 11S (e) likewise the ubiquitin-conjugating enzyme E2 (f) and ubiquitin itself (g) were also analysed by immunoblotting. Graphs from (b) to (g) represent bands quantified and normalised by GAPDH expression. Values are expressed as relative percentage of C group, referred as 100 %. Legend: C, control rats; W, Walker 256 tumour-bearing rats; M, metformin-treated rats (33 mg/Kg); WM, Walker 256 tumour-bearing rats treated with metformin (33 mg/Kg). Columns represent mean ± SD. *p < 0.05 vs C; #p < 0.05 vs W

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BMC Cancer

ISSN: 1471-2407

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