Fig. 1

PIK3CA exon 9 and exon 20 pyrograms (antisense strand). a Wild-type exon 9 sequenced with the 9-RS1 primer and the c.1634A > G mutation (arrow), causing a shift in the reading frame and a new peak at A (arrowhead), which serves as quality assurance. b Wild-type exon 9 sequenced with the 9-RS2 primer and the c.1624G > A mutation (arrow), causing a shift in the reading frame and a new peak at A (arrowhead). c Wild-type exon 9 sequenced with the 9-RS3 primer and the c.1633G > A mutation (arrow), causing a shift in the reading frame and new peaks (arrowheads). d Wild-type exon 20 sequenced with the exon 20 primer and the c.3139C > T mutation (arrow), causing a shift in the reading frame and a new peak at T (arrowhead). Wild-type exon 20 sequenced with the exon 20 primer and the c.3140A > G mutation (arrow), causing a shift in the reading frame and a new peak at G (arrowhead). Mut, mutant; WT, wild-type