Fig. 3

Ex vivo fluorescent imaging with gGlu-HMRG of two representative HNSCC cases (cases #1 and #5). a Endoscopic imaging with white light. b Narrow band imaging (NBI). c Iodine staining performed under general anesthesia. d Resected specimen observed with iodine staining. e Fluorescent imaging after spraying gGlu-HMRG. f Resected specimen mapping for tumor region. SCC was shown in red line. g Hematoxylin and eosin staining of the tumor and normal component. h Immunohistochemical examination investigating GGT expression in the tumor and normal component. Square lines in f correspond to the upper figures in g and h. Dotted square lines in f correspond to the lower figures in g and h. Scale bars of d-f, 1 mm (case#1) and 5 mm (case#5). Scale bars of g and h, 200 μm