Fig. 4From: GSK-3 directly regulates phospho-4EBP1 in renal cell carcinoma cell-line: an intrinsic subcellular mechanism for resistance to mTORC1 inhibitionIn vitro kinase assays. In vitro kinase assays were performed using recombinant GSK-3β and recombinant 4EBP1 as described in the Materials and methods. Either AR-A014418 (25 μM) or SB-216763 (25 μM) was applied in vitro to inhibit recombinant GSK-3β. p4EBP1 levels were measured with immunoblot analysis. Data are representative of three separate immunoblot analysisBack to article page