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Table 1 Summary of plasmid mixtures for electroporation

From: Induction of artificial cancer stem cells from tongue cancer cells by defined reprogramming factors

Mix-ture Plasmid name Amount (μg) Genes Resultant cell
1 pCXLE-EGFP 1 Egfp HSC2/EGFP
2 pCXLE-hOCT3/4-shp53-F 1 Oct4, shp53, Egfp HSC2/hOCT3/4-shp53-F
pCXLE-EGFP 1
3 pCXLE-hSK 1 Sox2, Klf4, Egfp HSC2/hSK
pCXLE-EGFP 1
4 pCXLE-hUL 1 l-Myc, Lin28, Egfp HSC2/hUL
pCXLE-EGFP 1
5 pCXLE-hOCT3/4-shp53-F 1 Oct4, shp53,Sox2, Klf4, Egfp HSC2/hOCT3/4-shp53-F + hSK
pCXLE-hSK 1
pCXLE-EGFP 1
6 pCXLE-hOCT3/4-shp53-F 1 Oct4, shp53, l-Myc, Lin28, Egfp HSC2/hOCT3/4-shp53-F + hUL
pCXLE-hUL 1
pCXLE-EGFP 1
7 pCXLE-hSK 1 Sox2, Klf4, l-Myc, Lin28, Egfp HSC2/hSK + hUL
pCXLE-hUL 1
pCXLE-EGFP 1
8 pCXLE-hOCT3/4-shp53-F 1 Oct4, shp53, Sox2, Klf4, l-Myc, Lin28, Egfp HSC2/hOCT3/4-shp53-F + hSK + hUL
pCXLE-hSK 1
pCXLE-hUL 1
pCXLE-EGFP 1