Fig. 5
From: Induction of artificial cancer stem cells from tongue cancer cells by defined reprogramming factors
Sphere formation assay. The transfectants and HSC2 cells were cultured in a sphere-culture medium. a The number of spheres was significantly higher in the HSC2/hOCT3/4-shp53-F + hSK + hUL than HSC2 and HSC2/EGFP. Error bars represent the standard deviation of the mean of three independent experiments. b Representative images of sphere (s) generated from each cell types (Bar  =  50 μm). The HSC2/hOCT3/4-shp53-F + hSK + hUL could also form bigger spheres