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Fig. 4 | BMC Cancer

Fig. 4

From: Macrophage phenotypic subtypes diametrically regulate epithelial-mesenchymal plasticity in breast cancer cells

Fig. 4

M1 macrophages accelerate mesenchymal to epithelial transition in MDA-MB-231. a Fluorescent imaging depicts MDA-MB-231 cells co-cultured with M0, M1 or M2 macrophages in serum-free hepatocyte maintenance medium (HMM) for 5 days. MDA-MB-231 cells mono-cultured in serum-free HMM on day 5 as the negative control, mono-cultured MDA-MB-231 in their normal growth medium (RPMI 1640 with 10 % serum) on day 5 as the positive control. (Bar = 10 μm). MDA-MB-231 phenotypic shift in a quantified by the ratio of the midpoint diameter divided by cell perimeter. Data are mean ± s.e.m of 3 independent experiments. *, P <0.05. **, P <0.01. b M1 macrophages drive the re-expression of E-cadherin in MDA-MB-231 breast cancer cells. Immunofluorescence analysis of E-cadherin expression in RFP-MDA-MB-231 cells by co-culture with M0, M1 or M2 macrophages. E-cadherin(green), RFP (red), DAPI (blue), Merge(yellow). (Bar = 10 μm). c Flow analysis of E-cadherin expression of RFP-MDA-MB-231 population after 5 days of co-culture with M0, M1 or M2 macrophages. The grey background represents unstained control. The black line indicates co-culture with M0 macrophages. The red line indicates co-culture with M1 macrophages. The blue line indicates co-culture with M2 macrophages. Shown is one of two similar experiments

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