Fig. 2

Basal growth rate of BT474 and MDAMB361 cells in 3D and 2D cultures. The basal growth of both cell lines (BT474 and MDAMB361) in control medium over a 7-day period in the 3D and 2D contexts were measured using coulter counting (a). There was a modest reduction in growth rate in 3D culture which was not statistically significant. Growth data by coulter counting was further validated by assessing expression of Ki67 (b). Again, no significant differences were observed between cells growing in 2D versus 3D culture