Skip to main content
Fig. 1 | BMC Cancer

Fig. 1

From: 3D culture of Her2+ breast cancer cells promotes AKT to MAPK switching and a loss of therapeutic response

Fig. 1

Breast cancer cells growing in 3D culture retain characteristics of 2D cultures. Bright field images of BT474 (a) and MDAMB361 (b) cells in 2D and 3D culture revealed that cells growing in 3D culture formed tightly packed spherical aggregates with a rounded (BT474) or grape-like (MDAMB361) appearance compared to 2D monolayers growth. Immunocytochemical staining of cellular receptors namely the Her2 and ER did not reveal any loss or change in cellular localization when cells were cultured in 3D compared with 2D (c, d)

Back to article page