Fig. 6

EphA2 is tyrosine phosphorylated by FVIIa and ephrin-A1. a MDA-MB-231 cells were treated with 10 nM FVIIa and samples were analyzed by Western blot for EphA2 phosphorylation on tyrosine 588 (Y588) and total EphA2 protein. FVIIa increased the phosphotyrosine signal to 110.0 ± 1.02 % (p < 0.001) after 30 min and 129.2 ± 9.82 % (p = 0.0067) after 60 min. N = 3, results are presented as means ± SD. b MDA-MB-231 cells were pretreated with 10 nM FVIIa for 1 h and then stimulated with 1 μg/ml ephrin-A1-Fc for 5 or 10 min. c MDA-MB-231 cells were pretreated with 10 nM FVIIa for 6 h and then stimulated with 1 μg/ml ephrin-A1-Fc for 5 min. Samples were analyzed by Western blot as in (a). N = 2–3, representative gels are shown