Fig. 1

Co-localization between TF and EphA2 in MDA-MB-231 cells. a EphA2 cleavage is independent of PAR2. MDA-MB-231 cells were pretreated with 50 μg/ml anti-TF antibody 10H10 or 100 μg/ml PAR2-blocking antibody for 30 min followed by 10 nM FVIIa for 6 h. Images show representative Western blots. The 10H10 antibody was verified to prevent IL8 mRNA induction by FVIIa (graph). MDA-MB-231 cells were pre-treated with 50 μg/ml 10H10 antibody for 30 min, and 10 nM FVIIa was added for 1 h. FVIIa increased IL8 mRNA to 224.6 ± 0.3 %, whereas preincubation with mab10H10 abolished induction to 112.6 ± 1.2 %. N = 2, results are presented as percent of untreated control with error bars indicating the standard deviation. b EphA2 and TF in proximity were detected in MDA-MB-231 cells using an in situ proximity ligation assay with antibodies towards TF and EphA2. Red signal corresponds to TF-EphA2 complexes, blue signal represents DAPI-stained DNA. c Confocal micrographs showing MDA-MB-231 cells stained for EphA2 (green signal) and TF (red signal). Blue signal represents DAPI-stained DNA. Scale bar represents 20 μm