Fig. 6

Images of 1.2 % denaturing agarose gels showing compound-induced cross-linking of double-stranded, PvuII-cleaved pUC19 DNA. The denaturing agarose gel images depict bands corresponding to the large (2364 bp long) PvuII-cleaved pUC19 DNA fragment following treatment with (a) cisplatin, (b) 1,8 platinum, (c) 1,10 platinum and (d) 1,12 platinum. The bands corresponding to the non-heat denatured dsDNA and heat denatured ssDNA forms (of the DMF control samples) are present on the left and right-hand side of the 1 kb ladder, respectively, of each gel. In each gel image, the bands to the right of the ssDNA control, correspond to heat-denatured DNA treated with increasing concentration of compound, ranging from 0.1 to 100 μM for cisplatin, and 0.01 to 10 μM for the dinuclear compounds (as indicated above each gel image)