Fig. 3

Images of LAR electropherograms showing DNA damage induced by cisplatin, transplatin and the novel dinuclear compounds in the PvuII-cleaved pUC19/T7 sequence. The top two electropherograms represent the (a) G sequence (generated via dideoxy sequencing and used as a reference for determining the location of all G-rich sites in the sequence) and the (b) 5 % DMF control (without drug). The following five electropherograms show a trace of peaks corresponding to DNA damage induced by (c) 0.3 μM cisplatin, (d) 0.3 μM transplatin, (e) 0.3 μM 1,8 platinum, (f) 0.3 μM 1,10 platinum and (g) 0.3 μM 1,12 platinum. For each electropherogram image, the relative fluorescence intensity of the peaks is plotted on the y axis and the relative DNA fragment size (bp) plotted on the x axis (shown at the top of the G sequence electropherogram). The peak corresponding to the full length extension product occurs at the expected size of 186 bp in all electropherograms, which is highlighted in the G sequence electropherogram. The primer annealing site occurs at the left of each electropherogram, as indicated in the 5 % DMF control. The sites of the seven telomeric repeats (T1 to T7) are indicated, as well as the other G-rich sites (G4, G3I, G3II and G5) at which DNA adducts occur