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Fig. 4 | BMC Cancer

Fig. 4

From: Methionine-restricted diet inhibits growth of MCF10AT1-derived mammary tumors by increasing cell cycle inhibitors in athymic nude mice

Fig. 4

Methionine restriction increases cell cycle inhibitors P21 and P27, while decreasing cyclin D1. a Murine cyclin D1 expression from mammary tissue near the MCF10AT1 tumor in CF and MR mice. N = 5 CF, =3 MR. b Murine P21 expression from mammary tissue sampled near tumor in nude mice on MR or CF diet. N = 9 CF, 7 = MR; c Expression of murine P27 from mammary tissue sampled near the tumor in nude mice on MR and CF diet. N = 7 CF, 7 = MR. d Real-time PCR on MCF10AT1 tumor from paraffin sections demonstrated a trend towards an increase in human P21 expression. e Real-time PCR on MCF10AT1 tumor from paraffin sections demonstrated a decrease in human P27 expression in the MCF10AT1 cells that comprise the human tumor (p = 0.09). D. and E. both had N = 5 animals each for CF and MR. f Increased P21 expression by real-time PCR in the breast cancer cell line MCF10A after growing cells in in methionine-restricted cysteine-depleted (MRCD) media for 24 h. g Increased P27 expression by real-time PCR in the breast cancer cell line MCF10A after growing cells in MRCD media for 24 h. h Increased P21 expression by real-time PCR in the breast cancer cell line MDA-MB-231 after growing cells in MRCD media for 24 h. i Increased P27 expression by real-time PCR in the breast cancer cell line MDA-MB-231 after growing cells in MRCD media for 24 h. f-i Cell culture experiments N = 4, with 3 replicates in each experiment. Mann-Whitney unpaired one-tailed t-test., ***p ≤ 0.001. ** p ≤ 0.01, * p ≤ 0.05

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