Fig. 2

Soluble E-selectin enhances migration of human leukocytes in vitro. a Effect of sE-selectin on the adhesion of leukocytes to non-activated p-HMVECs. A monolayer of p-HMVECs was grown on the parallel flow chamber. The cells (10⁵ cells/ml) were incubated with 100 nM sE-selectin for 30 min and then infused into the chamber at 37 °C over a 5 min period at a shear stress rate of 1 dyn/cm². Adhesion was expressed as a percent relative to adhesion of saline-treated control cells. b Migration assay was performed using Boyden chamber. HL-60 cells (5 × 10⁵ cells) were seeded in upper chambers (5-μm pores) and 100 nM sE-selectin was supplemented to the lower chambers. The number of migrated cells was counted after 16 h. Migration is expressed as a percent relative to saline-treated control. c Effect of CD44 knockdown of HL-60 on sE-selectin-induced adhesion. The data represent Mean ± S.D. *p < 0.05; **p < 0.01; ***p < 0.001, Student’s t test. Data were summarized as % of respective control from an experiment conducted in triplicate and repeated twice