Fig. 5

Inhibitors of HDAC1 increase BRM and VIM expression in a time-dependent manner. Total RNA was isolated from untreated SW13- cells (0 h), and SW13- cells treated with 0.15 μM MGCD0103, 0.51 μM MS-275, or 2 nM FK228 for 24, 48, and 72 h. Fold change in (a) BRM and (b) VIM mRNA expression from 0 h was determined by real-time PCR analysis using the 2^-ΔΔCt method normalized to GAPDH. Data are presented as mean ± SEM. Superscripts indicate statistical significance, p <0.05. Treatments that share the same superscripts are not significantly different from one another