Fig. 4

The ability of selected HDAC inhibitors to induce the switching from the SW13- to the SW13+ subtype. a Induction of vimentin expression was assayed at 24, 48, and 72 h using fluorescence intensity of vimentin normalized to DAPI staining at each time point (left columns). Dose response was measured by fold induction in BRM mRNA using qPCR for two doses of each inhibitor (right columns). b Representative immunofluorescence images which illustrate the range of vimentin induction in panel (a). Scale ranges from no detectable vimentin immunofluorescence signal (−) to + representing <20 %, ++ representing 20–40 %, +++ representing 41–60 %, ++++ representing 61–80 %, and +++++ representing 81–100 % of maximum vimentin normalized to DAPI staining. Images were taken using a 10× objective lens. Data (right hand column of panel a) are presented as mean ± SEM