Fig. 6

COX-2 inhibition attenuates H. pylori-induced beta-catenin upregulation in SGC7901 and MKN45 cells. a, b. COX-2 inhibitor NS398 attenuated H. pylori effects on cytoplasmic and nuclear beta-catenin protein expression. Confluent SGC7901 and MKN45 cells were pretreated with 50 μM NS398 for 2 h prior to culture with or without H. pylori for 12 h. Cytoplasmic and nuclear beta-catenin protein levels were then determined by western blotting. **P < 0.01 for H. pylori-infected versus control cells. c, d COX2 RNAi blocked the H. pylori induced upregulation of cytoplasmic and nuclear beta-catenin. Confluent SGC7901 and MKN45 cells were infected with pFU-GW-COX-2-shRNA for 72 h with or without H. pylori incubation for 12 h. Cytoplasmic and nuclear beta-catenin protein expression levels were then measured by western blotting. **P < 0.01 for MKN45⁺ H. pylori versus MKN45 control and COX2 siRNA⁺MKN45⁺ H. pylori cells