Fig. 3

COX-2 inhibition attenuates H. pylori-induced VEGF upregulation in SGC7901 and MKN45 cells. a The COX-2 inhibitor NS398 attenuated H. pylori-dependent VEGF mRNA induction. Confluent SGC7901 and MKN45 cells were pretreated with 50 μM NS398 for 2 h prior to culture with or without H. pylori for 12 h. VEGF mRNA expression relative to GAPDH mRNA was determined by quantitative RT-PCR. **P < 0.01 for H. pylori-infected vs control cells. b NS398 attenuated H. pylori induction of VEGF protein. SGC7901 and MKN45 cells were pretreated with 50 μM NS-398 for 2 h prior to culture with or without H. pylori for 24 h and 36 h. The culture supernatant was then analyzed by ELISA to determine VEGF protein content. **P < 0.01 for H. pylori-infected vs control SGC7901 and MKN45 cells. c RNAi-mediated COX2 inhibition blocked VEGF upregulation by H. pylori. Confluent SGC7901 and MKN45 cells were infected with pFU-GW-COX-2-shRNA for 72 h with or without H. pylori treatment for 48 h. VEGF protein content in culture supernatant was then measured by ELISA. **P < 0.01 for H. pylori-infected vs control cells