Fig. 2

Time-dependent VEGF induction by H. pylori in SGC7901 and MKN45 cells. a VEGF mRNA expression increased in H. pylori-treated cells. Confluent SGC7901 and MKN45 cells were incubated with 100 H. pylori bacteria/cell for 0, 6, 12, 24, and 36 h and then analyzed by real-time quantitative PCR to determine VEGF mRNA expression relative to GAPDH mRNA. **P < 0.01, 12 h versus 0 h in SGC7901 and MKN45 cells. b VEGF protein content was increased in H. pylori-treated cells. SGC7901 and MKN45 cells were incubated with H. pylori for 0, 6, 12, 24, 36, and 48 h, and then analyzed by ELISA to determine VEGF protein levels. **P < 0.01, 24 h versus 0 h in SGC7901 cells; ^△△P < 0.01, 36 h versus 0 h in MKN45 cells